Figure 3
From: Epigenetic Regulation of Androgen Receptor Gene Expression in Human Prostate Cancers
A, MSP analysis of primary and refractory PCas. Aberrant methylation of Regions 2 and 3 of the AR is detected in #P2 and #P3. In hormone-refractory tumor samples, aberrant methylation of Regions 2 and 3 of the AR is detected in tumor samples #R2, #R4, #R11, and #R12. B, Direct PCR sequencing of Region 1 of the AR in PCas. Bisulfate sequencing of a PCa sample (#P2) and a hormone-refractory PCa sample (#R11) is shown. Direct-sequencing profiles reveal methylation of three CpGs in both PCa samples. C, Bisulfite sequencing of the AR promoter region. Data for sample #P2 are represented. Methylated CpG sites are shown as filled circles and unmethylated CpG sites are indicated by open circles.
