Figure 3
From: Bcl-2 Family Gene Expression during Severe Hyperoxia Induced Lung Injury
Hyperoxia alters the expression of Bcl-2 family members. Total lung RNA (5 μg), isolated from mice exposed to hyperoxia (> 95%) for 0, 64, or 88 hours (white, gray, and black bars, respectively), was annealed to [32P] labeled antisense riboprobes (Probe - undigested probe, provides size marker), digested with RNase and separated by electrophoresis. A, Representative ribonuclease protection assay (RPA). Expected positions of protected products for each mRNA are indicated. Arrows depict the marked induction observed in Bcl-XL and Bax mRNAs. B and C, Cumulative data. Pro-apoptotic (B) and anti-apoptotic (C) Bcl-2 family mRNAs were quantified by phosphorimaging of RPAs, normalized to L32 mRNA content and expressed relative to room air exposed controls. Bars indicate mean ± standard deviation, n = 4–5 mice/group; * indicates p < 0.001 versus room air control; data representative of more than three experiments.
