Figure 4
From: Bcl-2 Family Gene Expression during Severe Hyperoxia Induced Lung Injury
Northern analysis of hyperoxia induced Bax and Bcl-XL mRNA expression. A, Total lung RNA (20 μg) isolated from mice exposed to room air (RA) or hyperoxia (O2) for 64 or 88 hours was electrophoresed, transferred to membrane, and hybridized with [32P]-labeled cDNAs for Bax, Bcl-XL, and L32. B and C, The hybridization intensity was quantified by phosphorimaging, normalized to the expression of L32 and graphed relative to the values of RA-exposed mice (means ± sd). Bcl-XL (B) and Bax (C) were increased at 64 and 88 hours of exposure to hyperoxia. * p < 0.001 vs RA.
