Figure 6
From: Bcl-2 Family Gene Expression during Severe Hyperoxia Induced Lung Injury
Bcl-XL, but not Bax, protein is induced by hyperoxia. Mouse lung was homogenized in lysis buffer following exposure to room air (0 hr) or > 95% oxygen for 84 hours. Western analysis of total protein (50 μg) for Bcl-XL, Bax, and actin as a loading control was performed as described in the “Materials and Methods” section. A, Representative Western analysis. B, Quantification of proteins by densitometry of Western radiographs, normalized to actin levels. Bars = mean ± sd; n = 3; * denotes p < 0.005 vs RA.
