Figure 7
From: Bcl-2 Family Gene Expression during Severe Hyperoxia Induced Lung Injury
Regulation of Bax, Bcl-XL, Bfl-1, and Bcl-2 in p53-deficient mice. p53 (+/+) (open bar) and p53 (−/−) (filled bar) mice were exposed to room air or hyperoxia for 84 hours. Total lung RNA (5 μg) was analyzed by RPA. The intensities of the protected fragments were normalized to the expression of L32 and graphed relative to the expression of the wild-type mice (mean ± sd; n = 3). A, Bax expression was significantly lower in room air exposed p53-null lungs versus p53 (+/+) lungs and was increased by hyperoxia only in the presence of p53 (* p < 0.05). B, Bcl-XL gene regulation was not significantly affected by p53-deficiency. C, Bfl-1 was decreased by hyperoxia only in p53 (+/+) mice (* p < 0.05). D, Bcl-2 was not affected by hyperoxia or p53 deficiency.
