Table 1 Quantification of Immunohistochemical Staining by Computerized Image Analysis

From: Effect of Angiogenic and Antiangiogenic Compounds on the Outgrowth of Capillary Structures from Fetal Mouse Bone Explants

Days of culture

Unskeletonized area (pixels/area)

Skeletonized area (pixels/area)

Crosspoints (number of pixels)

Endpoints (number of pixels)

7

24098 ± 29

7810 ± 2083

807 ± 164

197 ± 31

10

27284 ± 4247

10528 ± 1267

788 ± 282

350 ± 82

12

41904 ± 3880

13328 ± 450

1323 ± 144

386 ± 40

14

63484 ± 6198

20095 ± 2240

1835 ± 331

450 ± 74

  1. Seventeen-day fetal mouse metatarsals were cultured for 7, 10, 12, or 14 days. At the timepoints indicated, cultures were fixed and stained for platelet-endothelial cell adhesion molecule-1 (PECAM-1). Capillary outgrowth determined from quadruple cultures was quantified by computerized image analysis as described in “Materials and Methods.” The area of outgrowth in unskeletonized images was represented by the mean number of pixels per area (unskeletonized area). After the skeletonizing procedure, the total vessel length was indicated by the mean number of pixels per area (skeletonized area). The complexity of the vascular network was determined by the number of crosspoints and endpoints. Data are expressed as mean ± sem, n = 6 of triplicate experiments. A strong correlation between the unskeletonized area of outgrowth and the crosspoints (r = 0.9) and total vessel length (r = 0.93) was found.
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