Figure 2
From: Cell Lineage Analysis during Liver Development Using the spfash-Heterozygous Mouse
Development of bile ducts in wild-type mouse livers. A, Bile duct progenitors (arrow) at 15.5 days of gestation. Many hemopoietic cells are also seen. Hematoxylin-eosin staining. B, Cytokeratin immunostaining in 15.5-day liver. Precursor cells for biliary epithelial cells (arrow) around the portal vein express cytokeratins strongly. Hepatoblasts in other parts are weakly or moderately stained for the cytokeratins. C, OTC immunostaining in B. Precursor cells of biliary epithelial cells also express OTC. D, Squamous biliary epithelial cells (arrow) in 17.5-day fetal liver. Hematoxylin-eosin staining. E, Cytokeratin immunostaining in 17.5-day fetal liver. Cytokeratins are expressed strongly in biliary epithelial cells (arrow). F, OTC immunostaining in E. Biliary epithelial cells either express OTC or do not. PV, portal vein. Bar indicates 50 μm.
