Figure 2 | Laboratory Investigation

Figure 2

From: Expression of Nitric Oxide Synthases and Nitrotyrosine during Blood-Brain Barrier Breakdown and Repair after Cold Injury

Figure 2

Cold injury. Immunoreactivity for fibronectin (a and b) and eNOS (c to f) at the cold injury site is shown. a, Extravasation of endogenous fibronectin from lesion and perilesional vessels in the cold injury site at 2 days is shown. b, High magnification of perilesional vessels showing BBB breakdown to endogenous fibronectin. c, At 12 hours, mild eNOS immunoreactivity (1+) is present in endothelium of lesion vessels and in vessels in the surrounding brain. The margin of the lesion and surrounding brain is marked by arrows in c and d. At 2 days (d), there is a moderate (2+) increase in eNOS immunoreactivity in perilesional vessels, which appear dilated and tortuous. e, At day 4, perilesional vessels and profiles of neovessels show marked eNOS immunoreactivity (3+). f, At day 6, the lesion site is occupied by neovessel profiles that show moderate-to-marked eNOS immunoreactivity. The pial artery with marked medial hyperplasia overlying the lesion shows marked endothelial eNOS immunoreactivity. Magnification: a, × 30; b, × 130; c to f, × 250.

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