Figure 4 | Laboratory Investigation

Figure 4

From: Fibroblast Growth Factor 2 Activation of Stromal Cell Vascular Endothelial Growth Factor Expression and Angiogenesis

Figure 4

Left panel, VEGF and VEGF receptor-2/flk-1 expression in FGF-2–induced angiogenesis detected by in situ hybridization analysis. VEGF was detected with antisense probe (VEGF AS) and sense control (VEGF S) in FGF-2–containing Matrigel implants (7 days). Bright (B.F.) and dark inverted (D.F.) field photographs are presented. Arrows indicate strong focal cell staining. VEGF receptor-2/flk-1 was detected with antisense probe (FLK-1 AS) and sense control (FLK-1 S). Arrows indicate regions of positive staining. Dermis (D), stromal interface (I), and Matrigel (MG) are indicated. Right panel, Quantification of silver grains in the individual regions for VEGF antisense and sense and FLK-1 antisense and sense probes. The average of three fields in each region was measured and antisense compared with sense control for each region by Student's t test. * p < 0.005.

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