Figure 6 | Laboratory Investigation

Figure 6

From: Fibroblast Growth Factor 2 Activation of Stromal Cell Vascular Endothelial Growth Factor Expression and Angiogenesis

Figure 6

FGF-2–induced VEGF expression is independent of cell adherence to attachment matrices. A, Serum-free Balb/c 3T3 cells were plated onto serum- (Plastic), Matrigel- (ECM), and fibronectin (Fn)-coated plates in the presence (FGF-2) or absence (control) of FGF-2 (10 ng/ml) for 8 hours. Phase photographs denote attachment to all three matrices and activation by FGF-2 (increase in rounded cell morphology). B, Top panel, Northern blot analysis for VEGF and 36B4 control from cells on the indicated matrices in the presence (F) or absence (C) of FGF-2 (10 ng/ml) for 8 hours. Bottom panel, Phosphorimage quantification of the VEGF mRNA signal normalized to the 36B4 control for Balb/c 3T3 cells plated and treated on each indicated matrix. Note the nearly identical FGF-2–induced VEGF mRNA levels for each matrix.

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