Figure 4

A, Effects of N(G)-methyl-l-arginine (L-NMA; 10 and 100 μm) on maximal contractile response to PE in aortic rings taken from sham OVX and OVX rats treated daily with α-zearalenol (ZE; 1 mg/kg, im, in 100 μl of cottonseed oil), ZE plus ICI 182780 (2.5 mg/kg, im, in 100 μl of cottonseed oil), or vehicle (100 μl, im, of cottonseed oil). Each point represents the mean ± sem of six experiments. ♯ p < 0.001 versus OVX + vehicle; ^* p < 0.005 versus sham OVX and OVX + ZE. B, Effects of L-NMA (10 and 100 μm) on maximal contractile response to PE in aortic rings taken from sham OVX and OVX rats treated daily with 17β-estradiol (E; 20 μg/kg, im, in 100 μl cottonseed oil), E plus ICI 182780 (2.5 mg/kg, im, in 100 μl of cottonseed oil), and vehicle (100 μl, im, of cottonseed oil). Each point represents the mean ± sem of six experiments. ♯ p < 0.001 versus OVX + vehicle; ^* p < 0.005 versus sham OVX and OVX + E.