Figure 4

A, Content of IFNγ in protein lysates of specimens of normal pancreas (NP) and chronic pancreatitis (CP), as determined by an enzyme immunoassay. B to D, CD95L transcript and protein expression in AsPc1 and BxPc3 pancreatic carcinoma cells in the absence vs presence of 100 U/ml IFNγ for 48 hours. B, CD95L and SP1 mRNA detected in an ethidium bromide-stained agarose gel after RT-PCR. C, Ratio profiles of integrated optical densities. D, Immunoblot of Western blotted CD95L protein detected by CD95L monoclonal antibody G247-4 visualized by chemiluminescence