Figure 3

IL-11 does not stimulate Akt phosphorylation in HUVEC. Quiescent HUVEC were incubated with various doses of IL-11 and oncostatin M (positive control) for 15 minutes and analyzed for either Akt phosphorylation (P-Akt, ser473, upper panel), p42/p44 MAPK phosphorylation (middle panel, P-p42/p44, Thr202/Tyr204), or total Akt (lower panel) by immunoblotting as described in the “Materials and Methods.” Data are from one of two experiments with similar outcome.