Figure 4 | Laboratory Investigation

Figure 4

From: Interleukin-11 Up-Regulates Survivin Expression in Endothelial Cells through a Signal Transducer and Activator of Transcription-3 Pathway

Figure 4

Overexpression of dn-STAT3 and dn-STAT1 in HUVEC. HUVEC were transduced by retroviruses encoding either EGFP, dn-STAT3 (STAT3[Y705F]-FLAG), or dn-STAT1 (STAT1[Y701F]-HA) as described in “Materials and Methods.” Cell lysates were analyzed for either STAT3-FLAG (Fig. 6A, upper panel), total STAT3 (Fig. 6B, lower panel), STAT1-HA (Fig. 6B, upper panel), or total STAT1 (Fig. 6B, lower panel) by immunoblotting as described in “Materials and Methods.” Data are from one of two independent sets of transductants with similar outcome.

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