Figure 5 | Laboratory Investigation

Figure 5

From: Interleukin-11 Up-Regulates Survivin Expression in Endothelial Cells through a Signal Transducer and Activator of Transcription-3 Pathway

Figure 5

Effect of dn-STAT3 on phosphorylation of tyrosine 705 of endogenous STAT3 by IL-11. HUVEC were transduced by retroviruses encoding either EGFP or dn-STAT3 (STAT3[Y705F]-FLAG) and stimulated with various concentrations of IL-11 for 10 minutes. Cell lysates were analyzed for either STAT3 phosphorylation (P-STAT3, Tyr705, upper panel) or β-actin (lower panel) by immunoblotting as described in “Materials and Methods.” Data are from one of two independent sets of transductants with similar outcome.

Back to article page