Figure 3 | Laboratory Investigation

Figure 3

From: Potential Role of the Chemokine Receptors CXCR3, CCR4, and the Integrin αEβ7 in the Pathogenesis of Psoriasis Vulgaris

Figure 3

RT-PCR analysis of various chemokines in skin, cultured keratinocytes, and dermal endothelium. Expression of IP-10 (lanes 1 and 6), MIG (lanes 2 and 7), thymus and activation-regulated chemokine (TARC) (lanes 3 and 8), macrophage-derived chemokine (MDC) (lanes 4 and 9), and G3PDH, (lanes 5 and 10). In nonlesional skin, the only detectable chemokine mRNA was MDC (panel A, lane 4). In contrast, all four chemokines were detectable in lesional skin (panel A, lanes 6–10). In cultured keratinocytes, IP-10 mRNA was constitutively expressed (panel B, lane 1), and upon stimulation with TNF-α and IFN-γ, there was massive up-regulation of IP-10 (panel B, lane 6) and MIG (panel B, lane 7), and slight up-regulation of MDC (panel B, lane 9). In cultured dermal endothelium, none of the chemokine mRNAs were detected (panel C, lanes 1–4). Upon stimulation with TNF-α and IFN-γ, there was massive up-regulation of IP-10 (panel C, lane 6) and MIG (panel C, lane 7), and slight up-regulation of TARC (panel C, lane 8) mRNA.

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