Figure 1 | Laboratory Investigation

Figure 1

From: Expressional Regulation of Angiopoietin-1 and -2 and the Tie-1 and -2 Receptor Tyrosine Kinases during Cutaneous Wound Healing: A Comparative Study of Normal and Impaired Repair

Figure 1

Regulation of Ang-1, Ang-2, Tie-1, and Tie-2 mRNA expression during normal and diabetes-impaired wound repair. Expression of angiopoietins (Ang-1 and -2) and related receptors (Tie-1 and -2) during wound healing is shown for Balb/c control mice (A, normal repair, wt) and genetically diabetic db/db mice (B, impaired repair, db/db) as indicated. Total cellular RNA (20 μg) from nonwounded and wounded back skin of control mice (wt), or db/db mice was analyzed by RNase protection assay at the indicated time points after injury for the presence of Ang-1, Ang-2, Tie-1, or Tie-2 mRNA (left panels). Sixteen wounds (n = 16) from the backs of four animals were excised for each experimental time point and used for RNA isolation. ctrl skin refers to nonwounded skin of Balb/c and db/db mice. One representative experiment is shown; 1000 cpm of the hybridization probe were used as a size marker (probe). Expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA is shown as a loading control. The degree of regulation as assessed by PhosphoImager analysis of the radiolabeled gels is shown schematically in the right panels. For the right panels, note that every experimental time point represents a total of 32 wounds (n = 32) from two independent animal experiments.

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