Figure 2 | Laboratory Investigation

Figure 2

From: Expressional Regulation of Angiopoietin-1 and -2 and the Tie-1 and -2 Receptor Tyrosine Kinases during Cutaneous Wound Healing: A Comparative Study of Normal and Impaired Repair

Figure 2

Dysregulation of Ang-1, Ang-2, Tie-1, and Tie-2 protein expression during wound healing in db/db mice. Total protein (50 μg) from lysates of nonwounded and wounded back skin (Days 1, 3, 5, 7, 10, and 13 after injury, indicated at the top of each lane) isolated from Balb/c mice (wt) and diabetic mice (db/db) as indicated were analyzed by immunoblotting for the presence of Ang-1 (A), Ang-2 (B), Tie-1 (C), and Tie-2 (D) specific protein. Note that Tie-2 (D) represents an immunoprecipitation experiment. Eight wounds (n = 8) from the backs of four animals were excised for each experimental time point and used for protein isolation. For the individual animals shown in B and C (lower panels), four wounds each (n = 4) were pooled for analysis. ctrl skin refers to nonwounded skin of Balb/c and db/db mice. Ang-1, Ang-2, Tie-1, or Tie-2 protein is indicated by an arrow.

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