Figure 3 | Laboratory Investigation

Figure 3

From: Expressional Regulation of Angiopoietin-1 and -2 and the Tie-1 and -2 Receptor Tyrosine Kinases during Cutaneous Wound Healing: A Comparative Study of Normal and Impaired Repair

Figure 3

Localization of Ang-2 mRNA expression at the wound site. Paraformaldehyde (PFA)-fixed serial sections from 5-day mouse wounds isolated from Balb/c control animals were analyzed for the presence of Ang-2 mRNA expression by in situ hybridization. Sections were incubated with an FITC-labeled antisense oligonucleotide specific for Ang-2 mRNA (A to C). Note that directly neighboured sections (D to F) were incubated with a nonsense oligonucleotide as a negative control. All sections were stained with an alkaline phosphatase-coupled anti-FITC antibody using NBT/formazan as a chromogenic substrate. Nuclei were counterstained with hematoxylin. Scale bars are 100 μm for A and D, 50 μm for B and E, and 25 μm for C and F. Strongly immunopositive signals (A to C) and the corresponding negative controls (D to F) are indicated with arrows. g, granulation tissue; he, hyperproliferative epithelium; m, muscle; v, vessel.

Back to article page