Figure 5 | Laboratory Investigation

Figure 5

From: Expressional Regulation of Angiopoietin-1 and -2 and the Tie-1 and -2 Receptor Tyrosine Kinases during Cutaneous Wound Healing: A Comparative Study of Normal and Impaired Repair

Figure 5

Diabetes-impaired repair was characterized by reduced formation of CD31-positive vascular structures. Frozen sections (6 μm) from 5-day (Tie-2, wild-type) or 10-day (CD31, wildtype and diabetic) mouse wounds were incubated with polyclonal antisera directed against Tie-2 or CD31 as indicated. All sections were stained with the avidin-biotin-peroxidase complex system using 3-amino-9-ethylcarbazole as a chromogenic substrate. Nuclei were counterstained with hematoxylin. Scale bars are 25 μm for (Tie-2, wild-type), and 75 μm for (CD31, wild-type and diabetic). Strongly immunopositive signals are indicated with arrows.

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