Figure 6

Regulation of VEGF165 protein expression during normal and impaired healing. Total protein (50 μg) from lysates of nonwounded and wounded back skin (Days 1, 3, 5, 7, and 13 after injury, as indicated) isolated from Balb/c mice (wt) and diabetic mice (db/db) were assessed for the total amount of VEGF165-specific proteins during wound repair as determined by ELISA. Eight wounds (n = 8) from the backs of four animals were excised for each experimental time point and used for protein isolation.