Figure 7 | Laboratory Investigation

Figure 7

From: Expressional Regulation of Angiopoietin-1 and -2 and the Tie-1 and -2 Receptor Tyrosine Kinases during Cutaneous Wound Healing: A Comparative Study of Normal and Impaired Repair

Figure 7

Expression of lymphatic endothelium-derived VEGFR-3/Flt-4 mRNA during repair. Expression of von Willebrand factor (vWF) (A) and VEGFR-3/Flt-4 (B) during wound healing is shown for Balb/c control mice (wt) and diabetic mice (db/db) as indicated. Total cellular RNA (20 μg) from nonwounded and wounded back skin of Balb/c mice or db/db mice was analyzed by RNase protection assay at the indicated time points after injury for the presence of vWF or VEGFR-3/Flt-4 mRNA (upper panels). Sixteen wounds (n = 16) from the backs of four animals were excised for each experimental time point and used for RNA isolation. ctrl skin refers to nonwounded skin of Balb/c and db/db mice. One representative experiment is shown; 1000 cpm of the hybridization probe were used as a size marker (probe). The degree of regulation as assessed by PhosphoImager analysis of the radiolabeled gels is shown schematically in the lower panels. For the lower panels, note that every experimental time point represents a total of 32 wounds (n = 32) from two independent animal experiments.

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