Figure 2

RT-PCR detection of MSN-ALK transcript and cDNA breakpoint sequence. A, Nucleotide and amino acid sequence of the MSN-ALK cDNA junction. Nucleotides and amino acids are numbered from the beginning of the predicted MSN-ALK open reading frame. MSN sequence is in bold letters (Genbank accession number: AF295356). B, Only sample R922 showed the predicted MSN-ALK 298 bp band by RT-PCR analysis. RNA from ALCLs carrying the NPM-ALK (R257), TPM3-ALK (R891), and TFG-ALK (R789) translocations was used as a negative control and showed no evidence of the MSN-ALK transcript.