Figure 3

Temperature induced activation of platelets: static (A and B) and dynamic light scattering (C and D). In all four panels the same line types are used. The signals from platelets at 20° C are shown as dashed lines, from platelets at 37° C as full lines, and from platelets at 42° C as dotted lines. The normalized scattering curves of platelets at 20° C, 37° C, and 42° C are shown in Panel A. The pair distance distribution functions obtained from these scattering curves are shown in Panel B. The symmetry of the pair distance distribution function is indicative of the symmetry of the scattering particles. Spherical particles lead to a very symmetrical pair distance distribution function. The maximal distance denotes the largest dimension inside the scattering particles. Panel C shows the calculated volume weighted size distributions of platelets at 20° C, 37° C, and 42° C. The hydrodynamic radius (R_H) is an apparent size parameter calculated from the thermal motion. Increased friction reduces the thermal motion of the particles, which occurs when the scattering particles increase in size or change their shape. The electrophoretic mobilities (μ) for platelets at the three different temperatures are illustrated in Panel D. The results from dynamic light scattering (C and D) are summarized in Table 1.