Figure 7
Serotonin concentration in platelets and plasma. A, The average serotonin concentrations in plasma and the platelet pellet from three healthy donors at 37° C (black bars) and at 20° C (gray bars) in the presence of 5-hydroxytryptamine, serotonin (5-HT) re-uptake inhibitors, such as imipramine or fluoxetine are shown. An ELISA kit was used for the determination of plasma 5-HT, whereas the concentrations in the pellet were measured by high pressure liquid chromatography/absorbance detection. B, Histochemical detection of 5-HT in platelet-dense granules. Phase contrast and fluorescence micrographs show a discoid platelet at 37° C (a and b) and a platelet after temperature-induced shape change at 20° C (c and d). Serotonin is indicated by bright dots in the fluorescence micrographs caused by a secondary Texas Red-labeled antibody to a serotonin antibody.
