Figure 11 | Laboratory Investigation

Figure 11

From: Human Hepatocellular Carcinoma (HCC) Cells Require Both α3β1 Integrin and Matrix Metalloproteinases Activity for Migration and Invasion

Figure 11

Integrin α3β1 regulates MMP-2 activation and HCC invasion. The HLE scattering assay was performed in the presence of Ln-5 and anti-integrin functional monoclonal antibodies (30 μg/ml). The Ln-5 scattering effect was blocked by P1B5 (anti-α3) and by P4C10 (anti-β1) antibodies, whereas, in the presence of P1E6 (anti-α2) or GoH3 (anti-α6), cells scatter similar to the Ln-5 control. HLE growth in serum-free conditions was used as a control (SF) (original magnification, × 100) (Panel a). The same antibodies also inhibited HLE cell invasion through a reconstituted BM (Panel b). Conditioned media from cell scattering experiments were analyzed by zymography. P1B5 inhibits MMP-2 activation but GoH3 does not (Panel c).

Back to article page