Figure 5 | Laboratory Investigation

Figure 5

From: Human Hepatocellular Carcinoma (HCC) Cells Require Both α3β1 Integrin and Matrix Metalloproteinases Activity for Migration and Invasion

Figure 5

HCC cell adhesion on ECM proteins. HCC cells adhere strongly on Ln-1 and Coll IV (10 μg/ml), with some differences between each cell line. Chang, HLE, HLF, and SK-Hep1 adhere strongly on Ln-5 (1 μg/ml), whereas Hep3B, HepG2, and HepG2.2.15 do not. Alexander shows only weak adhesion. Wells coated with 10% BSA in PBS were used as a negative control. Experiments are quantified as the mean of three different wells ± sd, where adherent cells were stained and solubilized, and OD was measured with an ELISA plate reader.

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