Figure 2
From: Specific Deficiency in Nuclear Factor-κB Activation in Neurons of the Central Nervous System
A and B, Double immunofluorescence of neuron/glia cultures treated with lipopolysaccharide (LPS) and stained for both neurofilament (A) and NF-κB RelA (B). Arrow: Neurofilament positive neuron (A, green) with cytoplasmic RelA (B, red). Arrowhead points to neurofilament-negative astrocyte nuclei with characteristic oval profile visibly stained for RelA in B (see Fig. 3). Representative nuclear profiles are demarcated with dashed lines. C to F, In situ double exposure immunofluorescence of the RelA component of NF-κB and neuron-specific neurofilament proteins in neurons cultured from the mouse cerebral cortex treated with either control medium (C), cycloheximide (D), tumor necrosis factor-α (TNF-α) (E), or LPS (F).
