Figure 8
From: Specific Deficiency in Nuclear Factor-κB Activation in Neurons of the Central Nervous System
A, Astrocyte and neuron cultures were treated with (+) or without (−) CHX, plus 1 to 100 U/ml IFN-γ for 4 hours, and nuclear extracts were prepared. Nuclear proteins were used with the 32P-labeled duplex oligonucleotide IRF-1-GAS in an EMSA. B, The synergistic effect of cycloheximide and IFN-γ on IRF-1 mRNA expression. Northern blot analysis of astrocyte and neuron cultures treated with media alone, IFN-γ, CHX, or IFN-γ + CHX for 6 hours. C, Synergistic IRF-1 gene induction by IFN-γ and multiple protein synthesis inhibitors. Northern blot of neuron cultures treated with medium alone (Co), CHX (two separate lots), An, Pur, or Em alone, and in combination with 100 U/ml IFN-γ for 6 hours.
