Figure 1 | Laboratory Investigation

Figure 1

From: Regulation of the New Coexpressed CD55 (Decay-Accelerating Factor) Receptor on Stomach Carcinoma Cells Involved in Antibody SC-1–Induced Apoptosis

Figure 1

Expression of CD55 wild-type (CD55WT) and SC-1-specific CD55 isoform (CD55SC-1) on different cells. The expression of both CD55 isotypes were tested by Western blot and FACS analysis. For Western blot analysis, membrane extracts from 23132 and cervix carcinoma cell line (HeLa) tumor cells were run on SDS gels and blotted on nitrocellulose filters and either stained with anti-CD55 antibody or with SC-1. For FACS analysis, cells were additionally stained with mouse or human isotype-matched control antibodies. a, HeLa cells express two isoforms of CD55 of approximately 60 and 70 kd, whereas in 23132 cells the 70-kd and the SC-1–specific 82-kd form were detectable. SC-1 staining was not observable in HeLa cells, whereas staining of the 82-kd CD55SC-1 proteins was visible in the cell line 23132. Staining of additional protein is due to unspecific cross-reaction as described earlier. b, FACS analysis with anti-CD55 shows binding to cell lines 23132 and HeLa compared with the isotype-matched control. c, On cell line 23132, SC-1 shows binding compared with the isotype-matched control, whereas there is no binding found on HeLa cells.

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