Figure 4

Inhibition of caspase-6 and caspase-3 by specific inhibitors. Cells were incubated overnight with increasing amounts of caspase inhibitor and then for another 24 hours with 40 μg/ml of SC-1. Apoptotic cells were determined by CellDeath ELISA. a, Effect of caspase-6 inhibitor VEID-CHO. Low concentrations of inhibitor increase apoptosis, whereas high concentrations clearly inhibit apoptosis. b, Effect of caspase-3 inhibitor DEVD-CHO. An increase in apoptotic cells with increasing concentrations of inhibitor is observable, whereas incubation with only DEVD-CHO does not show any effect.