Figure 1 | Laboratory Investigation

Figure 1

From: Alterations on the 5′ Noncoding Region of the BCL-6 Gene Are Not Correlated with BCL-6 Protein Expression in T Cell Non-Hodgkin Lymphomas

Figure 1

A, Giemsa-stained paraffin sections (upper row) and frozen sections stained with the BCL-6 mAb (lower row) of the three peripheral T cell lymphoma (PTCL) cases with mutations of the 5′ noncoding region of the bcl-6 gene. A few scattered and faint BCL-6+ cells are detectable in Cases 20 and 26. Case 27 expresses the BCL-6 protein. B, Single strand conformation polymorphism (SSCP, upper row) and the sequence analyses (lower row) of the DNA samples from the same cases. Arrows indicate the abnormally migrating bands caused by nucleotide substitution in these regions. ND, nondenatured DNA; DNA from normal placenta was used as a wild-type sequence negative control (NC). P, polymorphic control; the numbers above each lane correspond to the case numbers. The lower row displays representative mutations detected by sequence analyses of these cases.

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