Table 1 DHPLC Conditions for Exons 5–8 of the p53 Gene
From: Denaturing High Pressure Liquid Chromatography (DHPLC) for the Analysis of Somatic p53 Mutations
Exon | Temperature (°C) | Acetonitril gradient (%B) |
|---|---|---|
5 | 62 | 50–63 |
65 | 48–60 | |
67 | 48–59 | |
6 | 61 | 48–61 |
7 | 64 | 47–59 |
8 | 59 | 49–61 |
62 | 47–59 | |
64 | 45–57 |
