Figure 7

Culture of immortalized podocyte cell line. A, Podocyte cell line grown at 33° C with 100 U/ml IFN-γ (left panel). Differentiated arborized podocytes at 37° C, no IFN-γ under normal glucose conditions (5.5 mm glucose; middle panel) or high glucose conditions for 3 days (25 mm glucose; right panel). Original magnification, ×150. B, ³H-thymidine incorporation (left) and ³H-leucin incorporation (right) into differentiated mouse podocyte cell line under normal glucose (NG), high glucose (HG), or normal glucose with mannitol (MN). Results represent means ± sem of six independent cultures. ^**p < 0.01 versus NG. C, Expression of p21, p27, p57, and β-actin. Equivalent amounts (10 μg/lane) of each sample were analyzed by Western blotting. Western blotting was independently performed five times. Representative data from one experiment are shown. Quantitative and statistical data are described in the text.