Figure 1
From: Abnormal Iron Deposition in Renal Cells in the Rat with Chronic Angiotensin II Administration
Iron deposition and heme oxygenase-1 (HO-1) expression in the kidney of angiotensin II-infused and control rats. A to E, G, and H, Prussian blue staining of iron in the kidney. F, Immunoperoxidase staining of HO-1. I. Turnbull’ s blue staining. Specimens were either from control rats (A) or from rats with angiotensin II infusion at a dose of 0.7 mg/kg/day for 7 days (B to I). Although no apparent iron deposition was seen in the control kidney (A), marked deposits of iron were observed in the renal cortex (B) but not in the medullary regions (C) of the kidney after angiotensin II infusion for 7 consecutive days. Iron deposits were seen occasionally in the cells of the Bowman’ s capsule (D). E and F were from serial sections. Intense HO-1 staining was observed in the renal tubular epithelial cells with positive iron deposition. G, Prussian blue staining of the kidney from angiotensin II-infused rats that were given zinc protoporphyrin, an inhibitor of HO. Iron deposition in the tubular epithelial cells could still be seen. H and I are from serial sections. A similar pattern of iron staining was demonstrated by Prussian blue and Turnbull’s blue staining. Original magnifications are × 100 (A to C, H, I), × 250 (E to G), and × 500 (D).
