Figure 5

A, schematically illustrates the trajectories of two cells with different types of behavior pattern. Although the first moved randomly and remained in a small area, the second established a more extended trajectory (about five times the cell size). Each cell trajectory was characterized by two parameters. The first was the average speed (the AS variable) defined by the length of the cell trajectory divided by the time taken by the cell to cover it, whereas the second was the maximum relative distance from the origin (the MRDO variable). This parameter is defined by the greatest linear distance between the reference point (t = 0) and any subsequent position of the cell (distances d1 and d2 in the figure) divided by the duration of the time of observation of the cell. B, documents the variation (black squares = median, lower/upper bars = lower/upper quartiles; ie, 25/75%) in the MRDO values observed during the entire experimental period (12 hours) for eosinophils plated on different supports (PLAS = plastic, P-Sel = 0.2 μg/cm² P-selectin, G-xx = xx μg/cm² galectin-1 with densities of 0.1, 1, and 10 μg/cm²). The levels of significance (p values) were computed in relation to the P-selectin–coated support. Similarly, C and D report the variation in AS values observed during the first and last 6 hours of the experiment, respectively.