Figure 1
From: Tissue Distribution and Induction of Human Multidrug Resistant Protein 3
Western blot detection of multidrug resistance protein (MRP) 3 protein in membrane fractions derived from normal human tissues (a and b). In b, adrenal gland and colon are shown from another, but essentially identical, Western blot experiment. As a positive control, a protein sample of MRP3-transfected 2008 ovarian carcinoma cells was loaded. MRP3 protein was visualized with monoclonal antibody M3II-9 and enhanced chemiluminescence. As observed earlier with tumor cell line–derived samples (Kool et al, 1999), differential glycosylation of MRP3 in human tissues results in doublet bands of slightly variable molecular weights.
