Figure 1 | Laboratory Investigation

Figure 1

From: Rho GTPase Inactivation Impairs Lens Growth and Integrity

Figure 1

A, Structure of the αA/C3 transgene. Bacterial C3 cDNA (coding region) containing a eukaryotic translation initiation sequence was subcloned into the polylinker region of the CPV2 plasmid between the αA-crystallin promoter and the SV40 small t antigen intron and polyadenylation sequences. The locations of primers used for screening genomic tail DNA and for RT-PCR analysis are shown in hatched and solid arrows, respectively. B, Sequence of the oligonucleotide representing the eukaryotic translation initiation site (large caps) inserted upstream of the C3 coding sequence (small caps). αAp = αA-crystallin promoter; ETIS = Eukaryotic translation initiation sequence; pA = Polyadenylation.

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