Figure 2

Expression of C3 transgene product. A, Total RNA extracted from a single pair or from pooled lenses from wild-type littermate controls and from OD04-11, ODO4-16, and ODO4-21 transgene-positive mice was used for RT-PCR analysis using C3-specific primers. Lanes 1 to 7 represent the following: 1, wild-type littermate lens; 2, ODO4-11 mouse lens; 3, −RT control to ODO4-11 lens sample; 4, ODO4-21 mouse lens; 5, ODO4-16 mouse lens; 6, nonlenticular ocular tissues of line ODO4-11; and 7, GADPH expression in nonlenticular ocular tissues of line ODO4-11 mouse. The expected 316-bp fragment was observed in line ODO4-11 and ODO4-21 lenses (lanes 2 and 4, respectively) but not in line ODO4-16 (lane 5) or in wild-type littermate lenses (lane 1). C3 transgene expression was found to be lens specific and showed no expression in nonlenticular ocular tissues (lane 6). Lane M indicates base pair marker. B, Expression of RhoA and RhoB in lenses of wild-type (lanes 1 and 2, respectively), transgenic line ODO4-11 (lanes 3 and 4), and line ODO4-21 mice (lanes 5 and 6). Lane 7 indicates −RT control. Lane M is base pair marker. These data indicate that RhoA and RhoB expression were not affected by the C3 transgene in line ODO4-11 and ODO4-21 mice.