Figure 5
From: Rho GTPase Inactivation Impairs Lens Growth and Integrity
a, Histologic changes in C3 transgenic mouse eyes. Whole eyes from wild-type littermates (A to D) and age-matched transgenic mice, embryonic (E15.5) (E), 4 day (F), 12 day (G), and 6 week (H) were fixed in glutaraldehyde as described in “Materials and Methods.” Sections were stained with hematoxylin and eosin. As the figure demonstrates, progressive degenerative histologic changes were observed in the C3 eyes beginning during embryogenesis. In eyes from neonatal and juvenile transgenic animals, such changes included ruptured lens capsule, severe vacuole accumulation, impaired fiber elongation and differentiation, and presence of nuclei in differentiated lens fibers. Arrows in panel F indicate hemorrhage in C3 transgenic eye. b, Histologic changes in C3 mouse lenses at higher magnification. Sections from the regions indicated with stars in Figure 5a were viewed at higher magnification to depict the abnormal changes in fiber cell organization, elongation, and differentiation. A to C and D to F are from control and transgenic samples, respectively, from E15.5, day 4, and day 12 mice. Arrows in panel F depict the presence of nuclei in fiber cells, indicating abnormal terminal differentiation. Data shown here are from line ODO4-21 mice, and similar changes were observed in line ODO4-11 mice (data not shown).
