Figure 5 | Laboratory Investigation

Figure 5

From: Mechanisms of Chronic Renal Allograft Rejection. II. Progressive Allograft Glomerulopathy in Miniature Swine

Figure 5

Acute allograft glomerulopathy on Day 18. IgM (A) and IgG (B) deposition is detected in glomeruli. Double staining with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling (TUNEL) (blue) and CD3 (brown) (C) or GS-IB4 (brown) (D) show that TUNEL+ cells do not express CD3 are GS-IB4+. Double staining with proliferating cell nuclear antigen (PCNA) (blue) and CD3 (brown) (E), GS-IB4 (brown) (F), or α-actin (G) demonstrate that PCNA+ cells do not express CD3 but express GS-IB4 or α-actin. Marked destruction of glomerular capillary network is indicated by the loss of GS-IB4 staining (H). A and B, ×600; C to G, ×800; H, ×700.

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