Figure 4

Change in ear thickness induced by injection with human chymase; 2.0 μg of human chymase (A) or human serum albumin (B) was injected intradermally to mouse ear at 7-day intervals and ear thickness was measured at 0, 1, 6, and 24 hours after each injection using a thickness gauge. Abilities of heat-inactivated chymase and saline to induce skin reaction were also examined. Increase in ear thickness was determined by subtracting the ear thickness before the first injection with human chymase from that of each point after. Closed square, human chymase (A) or human serum albumin (B); open square, heat-inactivated human chymase; open circle, saline. Data are mean ± sem ^*, p < 0.05; ^**, p < 0.01 as compared with the saline group (Student's t test, n = 5).