Figure 3 | Laboratory Investigation

Figure 3

From: Photocoagulation-Induced Retinal Gliosis Is Inhibited by Systemically Expressed Soluble TGF-β Receptor Type II via Adenovirus Mediated Gene Transfer

Figure 3

Effect of TGF-β on GFAP mRNA expression in cultured Müller cells. A, RT-PCR analysis with primers specific for GFAP (upper level) or β-actin (lower level) of RNA isolated from cultured Müller cells. Faint GFAP mRNA is expressed in Müller cells without stimulation. TGF-β stimulates mRNA expression in a dose-dependent manner. mRNA of GFAP was normalized by that β-actin and the ratio to the data is presented. The representative data of three experiments is presented. B, Semiquantitative data were generated by scanning film exposures of the gel using a densitometer. Three independent examinations were made and the results are expressed as a “fold” increase over the control GFAP mRNA expression (mean ± sd; * p < 0.05).

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