Figure 2
From: Variable Nuclear Localization of α-Catenin in Colorectal Carcinoma
Immunocytochemical detection of α-catenin in colorectal adenocarcinoma cell lines studied by confocal fluorescence microscopy. The nuclei appear red, being stained by propidium iodide, whereas α-catenin immunoexpression appears green, being stained by FITC (fluorescein isothiocyanate)-labeled antibody. A, Dispersed cells of the cell lines SW480, SW620, HCT116, HT29, and Caco-2 show cytoplasmic expression of α-catenin and punctate nuclear staining with larger puncta focally. In confluent monolayers of HCT116, HT29, and Caco-2, α-catenin is mainly localized in the cell membranes, and no nuclear staining is seen. B, A gallery of images taken at different levels of a cell from the cell line SW480 shows diffuse α-catenin localization in the cytoplasm and the nucleoplasm. There are focal areas of concentration of α-catenin in the nucleus. C, Side views show the level in the nucleus at which the image is taken, confirming that it is through the nucleus.
