Figure 1 | Laboratory Investigation

Figure 1

From: Highly Stable Fluorescent Nanocrystals as a Novel Class of Labels for Immunohistochemical Analysis of Paraffin-Embedded Tissue Sections

Figure 1

Electron microscopy and photostability of CdSe/ZnS nanocrystals. A, Electron microscopy photographs were recorded with the electron microscope (model JEM-100CX, with screen magnification 100,000; JEOL, Tokyo, Japan). Bar = 20 nm. B, Time-dependent photobleaching curves for CdSe/ZnS nanocrystals and the dyes Alexa Fluor488 and FITC recorded with the fluorescence microscope Nikon Diaphot 300. Samples of dilute nanocrystals or dyes were deposited and spread on glass coverslips. Continuous-wave excitation was provided by a Nikon super high-pressure mercury lamp HB10101AF (Nikon Company, Tokyo, Japan).

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