Figure 2 | Laboratory Investigation

Figure 2

From: The Amount of Immature Glial Cells in Organotypic Brain Slices Determines the Susceptibility to Murine Cytomegalovirus Infection

Figure 2

Immunohistochemical analysis of MCMV-infected brain slices using antibodies specific to neural cell markers and MCMV antigen. Brain slice cultures from 7-day-old mice were infected with mutant MCMV (RM461) and stained with X-Gal after 3 days of infection. X-Gal-positive cells (blue) around the ventricular walls were double-stained with antibodies to GFAP (A), nestin (B), and Musashi-1 (C) (brown). Double-stained cells show black in the cytoplasm (arrows). Immunohistochemical double-staining was performed using N2 antibody to MCMV immediate-early (IE) antigen 1 (blue) and the antibodies to GFAP, nestin, or Musashi-1 (brown). The viral antigen-positive cells were stained with the antibodies to GFAP (D), nestin (E), and Musashi-1 (F), but not MAP2 (G). Most of the X-Gal-positive cells (blue) around the subventricular zone were double-stained with the N2 antibody (red) (H). Most of the X-Gal-positive cells were not double-stained with antibody against an endothelial marker. Only a few were double-stained (arrow) (I). Original magnification, × 560 (A to C), × 400 (D to G), × 360 (H), × 90 (I).

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