Figure 3

Flow cytometric analysis of ST6Gal I and ligands for CD22, sialoadhesin, and sialylated TF on HuH7. HuH7 is negative for ST6Gal I surface expression. Cellular pretreatment with saponin reveals cytoplasmic expression of ST6Gal I. HuH7 is positive for CD22Rg and SnRg binding. HuH7 cells weakly bind mAb A78-G/A7 against TF and much stronger after cellular VCN treatment.