Figure 1 | Laboratory Investigation

Figure 1

From: Expression of Calnexin Reflects Paneth Cell Differentiation and Function

Figure 1

Characterization of the anti-calnexin (Cnx) antibody. A and B, Immunoscreening experiments. A, Second step in plaque purification: clones expressing Cnx (black) and clones without any Cnx expression (white) exist together on a filter (ECL staining). B, Last step in plaque purification: one isolated clone expressing Cnx is shown (black; ECL staining). Note, white points in the periphery are used for filter orientation. C, One-dimensional SDS-PAGE of eluted proteins after immunoaffinity chromatography (left). The single reaction product of approximately 85 kDa shows immunoreactivity against Cnx in Western blot analysis (right). D, The 85-kDa protein isolated from the SDS-PAGE gel is identified as human Cnx by matrix-assisted laser desorption/ionization (MALDI)-PSD fragment ion analysis of the parent ion at m/z 1770.60 (arrow; BC003552). E, Isotype-specific mapping reveals that the mAb against Cnx is a rat IgG2a subtype.

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