Figure 2 | Laboratory Investigation

Figure 2

From: TNF-α Protects Human Primary Articular Chondrocytes from Nitric Oxide-Induced Apoptosis Via Nuclear Factor-κB

Figure 2

TNF-α inhibits SNP-induced DNA fragmentation. Chondrocytes were pretreated or not with TNF-α (10 ng/ml), for 24 hours. SNP (1 mm) was then added for an additional 24 hours. Genomic DNA isolation and 32P labeling were performed as in “Materials and Methods” to detect fragmented high-molecular weight (HMW) DNA (Relic et al, 2001). Lane 1, control cells; lane 2, SNP-treated cells; lane 3, TNF-α pretreated, SNP-treated cells.

Back to article page