Figure 6
Adenovirus-mediated IκB-α overexpression inhibits the TNF-α antiapoptotic effect. A, NF-κB-DNA binding activity in nuclear extracts from chondrocytes infected with adenovirus expressing mutated nondegradable IκB-α (Ad5-IκB) or control Ad5-GFP adenovirus for 24 hours, in the absence of any stimulus or before TNF-α (10 ng/ml) treatment for 24 hours. The arrow indicates the p65/50 complex. B, Chondrocytes were infected with Ad5-IκB for 24 hours, before 24 hours of TNF-α (10 ng/ml) treatment. SNP was then added for an additional 24 hours. Cell survival was estimated by MTS test, and results were expressed as percent of surviving cells compared with control untreated cells (100%). *a, Statistically different from the nontreated control (p < 0.05); *b, statistically different from SNP-treated cells in the absence of TNF-α (p < 0.05); *c, statistically different from SNP-treated cells in the presence of TNF-α (p < 0.05); *d, statistically different from SNP-treated cells in the presence of AdIkB and TNF-α (p < 0.05).
